Remote Ischemic Preconditioning (RIPC) modifies the plasma proteome in children undergoing repair of tetralogy of fallot: A randomized controlled trial

M Hepponstall; V Ignjatovic; S Binos; C Attard; V Karlaftis; Y D'Udekem; P Monagle; IE Konstantinov

Journal article

Remote Ischemic Preconditioning (RIPC) modifies the plasma proteome in children undergoing repair of tetralogy of fallot: A randomized controlled trial

M Hepponstall, V Ignjatovic, S Binos, C Attard, V Karlaftis, Y D'Udekem, P Monagle, IE Konstantinov

Plos One | Published : 2015

DOI: 10.1371/journal.pone.0122778

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Abstract

Background: Remote ischemic preconditioning (RIPC) has been applied in paediatric cardiac surgery. We have demonstrated that RIPC induces a proteomic response in plasma of healthy volunteers. We tested the hypothesis that RIPC modifies the proteomic response in children undergoing Tetralogy of Fallot (TOF) repair. Methods and Results: Children (n=40) were randomized to RIPC and control groups. Blood was sampled at baseline, after cardiopulmonary bypass (CPB) and 6, 12 and 24h post-CPB. Plasma was analysed by liquid chromatography mass spectrometry (LC-MS) in an untargeted approach. Peptides demonstrating differential expression (p <0.01) were subjected to tandem LC-MS/MS and protein identifi..

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University of Melbourne Researchers

Vera Ignjatovic Author

Vicky Karlaftis Author

Yves D'Udekem D'Acoz Author

Paul Monagle Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

The project was funded by the National Health and Medical Research Council (NHMRC) grant (#628756) and NHMRC postgraduate scholarship (#1017734). The study was supported by the Victorian Governments's Operational Infrastructure Support Program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.